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Presenter:  Elizabeth Duran

Discussion summary:

We discussed Maillard, RA et al. "ClpXP Generates Mechanical Force to Unfold and Translocate its Substrates", Cell 145, 459, 2011.  A single-molecule study using optical tweezers showed that ClpX is able to generate mechanical force to unfold substrates, and favored a power-stroke mechanism for the motor.  Another interesting observation was that the group was able to observe a well-defined intermediate in the  unfolding of GFP by ClpX and define the location of the intermediate.  This is an important step forward in research on AAA+ unfoldases.

Points that were discussed:

  • Definition of rips
  • Evidence for the power stroke model
  • How the molecules are trapped by optical tweezers